Abstract : Objective: To observe intraoperative practices adopted by the Surgical Staff regarding Surgical Site Infection Prevention as per WHO guidelines in Private Sector Tertiary Care Hospitals of Peshawar. Methodology: This was an observational study which included surgical staff of Peshawar, conducted from May 2022 to July 2022. Participants were selected by purposive serial technique and their surgical infection prevention control practices were observed by investigators in operation theatre. The data was analysed using SPSS version-25. The categorical variables like frequency and percentages were calculated. Results: Surgical staff members were assessed for their compliance to WHO guidelines regarding surgical site infection prevention control. The frequency of guidelines practiced/adopted by participants was not satisfactory. Conclusion: According to observational analysis of our study, the surgical site infection prevention practices adopted by the surgical staff of private sector tertiary care hospitals of Peshawar were not according to the WHO guidelines. As health care professionals they should not only primarily know about the updated guidelines issued by WHO, but also effective workshops should have to be carried out to acknowledge them about the national recommendations regarding surgical site infection prevention control. The national economic burden of nosocomial infections resulting after surgeries should also be highlighted accordingly.