Abstract : Introduction:.Various diagnostic modalities for varicose vein are Doppler USG. Pethysmography, venography. By HR MAS NMR spectroscopy metabolic environment of varicose veins can be studied and findings can be utilised in identification of new markers in pathogenesis, diagnosis and treatment..This research work is to study the metabolic environment of varicose veins by HR MAS NMR Spectroscopy and identification of a diagnostic marker and pathway of disease pathogenesis. Methods: Serum (n=37) and vein tissue (n=30) taken from varicose vein patient and analysed with HR MAS NMR Spectroscopy and compare with serum (n=79) and vein tissue (n=14) of non-varicose patient. Results: Higher concentrations of lipoproteins found in serum and of metabolites of lipids and proteins (glutamate, alanine, creatine and myoinositol) in venous wall samples of varicose vein patients as compared to non varicose patients. Conclusion: Higher concentrations of lipoproteins in serum may be suggestive of role of lipoproteins in diagnosis and of lipid lowering agents in treatment of varicose vein. Higher concentrations of metabolites of lipids and proteins in vein tissue indicates an ongoing inflammatory process thus anti-inflammatory drugs may have a role in treatment of disease.