The nanoparticles have various benefits in the biomedical field. The present research aimed to synthesize the copper nanoparticles from siddha therapeutics poly-phyto Vallarai Chooranam and its biomedical applications of antibacterial and anticancer properties. A copper nanoparticles (Cu NPs) process was carried out utilizing vallarai chooranam (VC) as a biological reducing substance as well as the UV–visible spectra at 432 nm were used to confirm the Cu NPs. Proteins as well as other ligands were found to be responsible for the capping of produced Cu NPs in UV-Visible spectroscopy (UV). Further, X-ray Diffraction (XRD) and Transmission Electron Microscopy (TEM) explored the crystalline nature of spherical morphological particles with a size range of 40 and 50 nm. An average diameter of Cu NPs was identified as 204.6 nm utilizing Dynamic Light Scattering (DLS) analysis as well as the zeta potential was – 141.9 mV, indicating that VC-Cu NPs are stable. The antimicrobial activity of VC-Cu NPs against Bacillus subtilis demonstrated a maximum zone of inhibition (14 mm). Cu NPs strongly reduced MCF-7 cell growth, by an IC50 score of 3.44088+/-230.7(6703 percent) µg/ml of the concentration in the minimum tested concentration (10µg/ml), Cu NPs were able to suppress the cell line's development by lesser than 10%. The inclusion of 50µg/ml Cu NPs, on the other hand, dramatically slowed the cell line's development (by >93%). We showed that a simple, effective, and fast synthesis process of Cu NPs can be utilized to create vast quantities of Cu NPs for use in biomedical applications.