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Abstract : Abstract: Introduction: Global health issue of COVID-19's rapid spread has a substantial impact on educational institutions. Therefore, the COVID-19 pandemic has altered the way we learn in high education and compelled us to convert to an online learning environment, the current study set out to assess students' stress of online education during the COVID-19 period. Method: This study used the sampling approach, where a simple Random sample was collected, and 500 students were selected out of five colleges in the universities of Kerbala. A three-part questionnaire was used to achieve the study objectives. The first section contains social and demographic information for the student, the second section contains academic variables related to the use of smart devices and electronic problems, and the third section contains psychological variables related to assessing students' stress according to PSS-10. Result: The mean for age participants was ( 22± 2) years old. The results showed that around (64.6%) of students had high levels of stress, while approximately (34%) had moderate levels. also about (1.4%) had a low level of stress through e-learning during COVID-19. Most participants were female (67.8%), There is a significant association between stress level and college, (24.1%) of higher-stress levels in candidates of Engineering college and (16.4%) of lowest-stress levels in candidates of College of Education and Physical Sports. there is a significant difference between electronic problems and the income of students, students with poor income were more likely to have online learning problems. p-value < (0.05) is significant. Conclusion: more than half of our students had a high level of perceived stress, and more than one-fourth had a moderate level of stress among the students of Kerbala University after the outbreak of Covid-19 disease. This study found a significant association between stress levels and college. Keywords: perceived stress, COVID-19, Distance learning, college student